Subdiffraction imaging
Web6 Oct 2013 · 4. Three-dimensional subdiffraction-limited imaging of a living cell. To demonstrate the spatial resolution improvement in the lateral axis (x–y plane), we … WebAbstract Recently, we introduced a new switching-off technique applicable to subdiffraction-limited coherent Raman imaging, where a coherent anti-Stokes Raman scattering (CARS) signal can be selectively suppressed via competitive stimulated Raman scattering (SRS) processes between vibrational modes of a single molecular species.
Subdiffraction imaging
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Web8 Oct 2016 · 蛋白质常常以复合体形式发挥功能。例如，包括g蛋白在内的许多信号转导蛋白以异三聚体形式行使功能。在细胞内原位监测多蛋白复合体，对其功能解析极为重要，但一直存在方法 ... Web15 Feb 2013 · We demonstrate quantitative multicolor three-dimensional (3D) subdiffraction imaging of the structural arrangement of fluorescent protein fusions in …
Web20 Apr 2016 · Toward Long-Distance Subdiffraction Imaging Using Coherent Camera Arrays. Abstract: In this work, we propose using camera arrays coupled with coherent illumination … WebCondensates vary from subdiffraction-limited sizes to a few microns (Keber et al. 2024, Shin & Brangwynne 2024). Live cell fluorescence microscopy is commonly used to visualize cellular condensates with at least one fluorescently labeled component. ... Combining high-resolution imaging with 3D DNA fluorescence in situ hybridization revealed ...
Web5 May 2015 · Here, we present a three-dimensional multimodal sub-diffraction imaging technique with spinning-disk (SD) confocal microscopy called 3D-MUSIC, which not only … Web13 Dec 2024 · Some studies have revealed that the diffraction of light using a binary mask produces a subwavelength spot that allows imaging with a higher resolution than that of …
Web12 Apr 2024 · The initial and inscribed polymer samples were characterized regarding potential laser-induced surface ablative modification by atomic force microscopy (AFM, Figure 3), using a scanning probe microscope Certus Standard V (Nanoscan, Moscow, Russia) in a contact mode. 3D imaging of bulk buried diffraction gratings, bulk photo- or …
Web10 Jan 2014 · Subdiffraction optical microscopy allows the imaging of cellular and subcellular structures with a resolution finer than the diffraction limit. Here, combining the … elize brazilWeb10 Jan 2014 · Our method is based on a double-excitation process, where the first excitation pulse partially and inhomogeneously bleaches the molecules in the diffraction-limited … tee erasmusWebBreaking Abbe’s diffraction resolution limit in fluorescence microscopy - Read online for free. elize kooijWebBy an iterative process in which a few molecules are activated, imaged and then switch-off or photo-bleached, a sub-diffraction image can be reconstructed after determining the … elizawashere spanjeWebA method for subdiffraction imaging that accumulates points by collisional flux. In PAINT imaging, the imaging target is continuously targeted by fluorescent probes in the solution. Each frame records the single-molecule fluorescence emitted by the nonspecific interaction between the freely diffused fluorescent probe and the target surface. tee gas lineWebnanolithography and nano imaging are also presented far field optical imaging with subdiffraction resolution January 23rd, 2024 - wei j nonlinear super resolution nano optics and applications ch 1 1 12 springer 2015 johnson t w et al highly reproducible near field optical imaging with sub 20 nm tee für prostataWebStudying the structural edits occurring during diseases of the nervous system requires imaging heterogeneous cell populations at an circuit, handy and subcellular degrees. Recent advancements in brain tissue clearing and development methods allow exceptional detailed visualization of the nervous system through its entire scale, of current to synapses, … elizazu