2024 Pacc antibody

Pacc antibody

Author: uxla

August undefined, 2024

WebAug 31, 2012 · The N. crassa pac-3 knockout strain (pac-3 KO ) was generated by replacing the pacC open reading frame (ORF; NCU00090) with the bar gene from a mus-52 KO background strain. Gene knockout in the... WebPacC may act either as a positive or negative regulator of secondary metabolites biosynthesis (Brakhage, 2013). Similar to our findings, PacC was found to serve as a …

PacC and pH–dependent transcriptome of the mycotrophic fungus

WebJun 13, 2014 · pACC expression in patients with gastric cancers We then detected the expression of inactive form of ACC (pACC) in normal and malignant epithelium. pACC-positive staining was preferentially cytoplasm localized determined by both immunofluorescence assay and immunohistochemistry. WebJul 25, 2011 · Thirteen of fifteen (87%) AICAR-responding neurons were immunostained positively with the antibody against phosphorylation of Ser79 of ACC (pACC) (Fig. 2 C and D). On the other hand, only one of 18 (6%) of the neurons not responding to AICAR were immunostained positively with the pACC antibody. holi 2022 date punjab

Ambient pH Controls Glycogen Levels by Regulating Glycogen

WebThe phosphorylation of AMPK and ACC was immunoblotted with anti-phospho-Thr-172 AMPKα (α-pAMPK) and anti-phospho-Ser-79 ACC (α-pACC) antibodies, respectively, and the expression of total AMPK protein was reprobed with anti-AMPKα2 antibody (α-AMPKα2). B, dose-response effect of metformin on AMPK and ACC phosphorylation. The … WebSep 30, 2015 · Our findings indicate that P. expansum secretes significant levels of ammonia at the leading edge of the colony, temporally modulating the activation of the transcription factor pacC (which modulates pH response factors). The activation of pacC under acidic conditions was confirmed using antibodies against different parts of the … WebAntibody FAQs: Storage: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. Value-added Deliverables: ① 200ug * antigen (positive control); ② 1ml * Pre-immune serum … holi 2022 date rajasthan in hindi

The preclinical Alzheimer cognitive composite: measuring …

How to solve problems with detecting pACC and ACC by

WebPhospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody … WebDoes anybody have experience with ACC (Acetyl Coenzyme A Carboxylase) and pACC detection by western blotting? It is often used to confirm the status of AMPK activity. The antibodies we use are... fat az gliderWebPhospho-Acetyl-CoA Carboxylase (Ser79) Antibody (PA5-117189) in WB Western blot analysis of extracts from 293 cells using Phospho-Acetyl-CoA Carboxylase (Ser79) Polyclonal Antibody ( Product # PA5-117189 ). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. holi 2022 thakur prasad calendar

"WebApr 11, 2024 · Adenosine monophosphate-activated protein kinase (AMPK) is the major sensor and regulator of cellular-energy metabolism. 6 AMPK, which is a serine (Ser)/threonine (Thr) kinase complex, consists of α catalytic subunits (α1, α2), β scaffolding subunits (β1, β2) and γ regulatory subunits (γ1, γ2, γ3). 6, 7 Phosphorylation of Thr172 … " - Pacc antibody

Pacc antibody

Augmented AMPK activity inhibits cell migration by …

WebThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Target Information … WebFeb 28, 2013 · Construction of loss- and gain-of-function mutants in the pH-sensitive regulator pacC T. virens pacC gene. Having initiated this study before the T. virens …

Did you know?

Web(B) PACC shows the same molecular mass at pH 5.8 and pH 7.8. Crude cellular extracts prepared from wild-type N. crassa submitted or not to alkaline pH stress were analyzed by Western blotting using a polyclonal anti-PACC antibody. The protein α-tubulin (theoretical molecular mass 50 kD) was used as a loading control. WebOct 20, 2024 · Introduction Acinar cell carcinoma (ACC) is an epithelial tumor similar to the acinar cells of the exocrine gland ( 1 ). It can originate from the pancreas, salivary gland, prostate, and lung ( 2 ), among which pancreatic acinar …

WebAnti-PACC1/TMEM206 (extracellular) Antibody (#ACL-031) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used … WebWhere polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are …

WebThis antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]. The Life … WebDec 23, 2011 · The membranes were rocked gently in blocking buffer (Li-Cor Bioscience, Lincoln, NE) and probed with primary antibodies at 4 °C overnight to eEF2K, phospho-eEF2 at Thr56, eEF2 and phospho-acetyl-CoA carboxylase (pACC) at Ser79 (Cell Signaling Technology, Danvers, MA). The antibody against β-actin was from Sigma.

WebRabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) RIM101 Polyclonal antibody. Uniprot No. P33400. Target Names. RIM101. Alternative Names. RIM101 antibody; RIM1 antibody; YHL027W antibody; pH-response transcription factor pacC/RIM101 antibody; Regulator of IME2 protein 1 antibody; pH-response regulator …

WebFeb 7, 2024 · To investigate whether AMPK might be responsible for the protective effects of metformin, hepatic AMPK activity was assessed by determining the phosphorylation levels of AMPK and its downstream... fataz muskyWebOct 16, 2014 · Author Summary Inhaled spores of the pathogenic mould Aspergillus fumigatus cause fungal lung infections in humans having immune defects. A. fumigatus spores germinate within the immunocompromised lung, producing invasively growing, elongated cells called hyphae. Hyphae degrade the surrounding pulmonary tissue, a … fat az muskieWebJan 30, 2015 · Both the S175A and S177A mutants lost cross-reactivity with pACC antibody (Fig. 2a). Next, we performed in vitro phosphorylation assays on recombinant FLAG-tagged Pdlim5 (WT, S175A and S177A). The results revealed that incorporation of [γ- 32 P]ATP into Pdlim5 was inhibited only in the S177A mutant, indicating that Ser177 is the unique ... holi 2023 thakur prasad calendarWebAcetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of … fat az musky luresWebFeb 8, 2008 · Immunocytochemistry of phospho-AMPK-α (pAMPK) and phospho-ACC (pACC) was performed as follows. Cells were pretreated with 3% H 2 O 2 in distilled water for 10 min and blocked with 10 mM Tris–HCl buffer (pH 7.5) containing 100 mM NaCl, 0.1% Tween 20, and 5% NGS for 1 h. holi 2023 date mumbai kalnirnayWebApr 25, 2014 · The following primary antibodies were used for IHC staining: pAMPK (Thr 172), pACC (Ser 79), and phosphorylated extracellular signal-regulated kinase (pErk) (Thr 202/Tyr 204) (All Cell Signaling Technology; Danvers, MA, USA) and immunoglobulin G (clone SP3) (Thermo Fisher Scientific, USA). fat az raptorWebPhospho-Acetyl-CoA Carboxylase (Ser79) Antibody (PA5-117189) in WB. Western blot analysis of extracts from 293 cells using Phospho-Acetyl-CoA Carboxylase (Ser79) … holi 2023 date in bihar