WebFlow: 1 ml/min (HiTrap 1 ml), 5 ml/min (HiTrap 5 ml), 3 ml/min (HiLoad 20 ml), 8 ml/min (HiLoad 53 ml) or at 50–100 cm/h for Sepharose High Performance packed in larger columns. Collect fractions throughout the separation. Equilibrate column with 5–10 column volumes of start buffer or until the baseline, eluent pH and conductivity are stable. WebPoorly packed bed (not sufficiently compressed during packing). (Not applicable for prepacked columns) Evaluate the packing using recommended methods. Please be …

HiTrap MabSelect SuRe, 1 x 1 ml Cytiva

WebNo. supplied 5 × 1 ml 100 × 1 ml1 5 × 5 ml 100 × 5 ml1 Special pack size delivered on specific order. Connector kit Connectors supplied 1/16” male/ luer female Tubing connector flangeless/ M6 female Usage Connection of syringe to top of HiTrap column Connection of tubing (e.g. Peristaltic Pump P1) to bottom of HiTrap column1 No. supplied 1 1 WebSample volume: 0.3 ml, 1.3 ml, 2.2 ml Eluent: 50 mM sodium phosphate buffer, 0.15 M sodium chloride, pH 7.0 Flow rate: 5 ml/min Detection: UV (280 nm, 5 mm cell) and … mcdonalds genesee st buffalo ny

HiTrap SP HP, 1 ml and 5 ml HiTrap Q HP, 1 ml and 5 ml

WebTable 1. Characteristics of HiTrap columns. Note: The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography … WebHiTrap ® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap ® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer … Web(0.1 M sodium acetate, pH 6.0 at 1 ml/min) Q: approx. 50 mg HSA/ml medium (20 mM Tris-HCl, pH 8.2 at 1 ml/min) Mean particle size 34 µm Bead structure 6% highly cross-linked … mcdonalds.gift card balance